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CMS PROPOSES DEFINITION OF MEANINGFUL USE OF CERTIFIED ELECTRONIC HEALTH RECORDS (EHR) TECHNOLOGY

December 31st, 2009 by admin

Beyond the Stage 1 Criteria for Meaningful Use

The policy goals of meaningful use will be most fully realized by building on findings from Stage 1 and by making full use of the greater proliferation of certified EHR technology and supporting HIT infrastructure that will take place under Stage 1.  CMS intends to propose through future rulemaking two additional stages of the criteria for meaningful use.

Stage 2 would expand upon the Stage 1 criteria in the areas of disease management, clinical decision support, medication management, support for patient access to their health information, transitions in care, quality measurement and research, and bi-directional communication with public health agencies.   CMS may consider applying the criteria more broadly to both the inpatient and outpatient hospital settings. 

Consistent with other provisions of Medicare and Medicaid, Stage 3 would focus on achieving improvements in quality, safety and efficiency, focusing on decision support for national high priority conditions, patient access to self management tools, access to comprehensive patient data, and improving population health outcomes.

Additional information can be found at www.cms.hhs.gov/Recovery.

CMS provides a 60-day comment period on the proposed rule.  The proposed rule may be viewed at http://www.cms.hhs.gov/Recovery/11_HealthIT.asp.

Notice “support for patient access to their health information” happens in stage 2 of the meaningful use criteria.

Good news, it’s included in some capacity…and this gives all of us consumer-centric co’s time to build.

Bad news, the NHIN will march onward focused on stage 1, which means we’ll be scrambling for simplification when it comes time to integrate patient capabilities to access data.

Buckle your seatbelts boys and girls – it’s gonna be a loooong trip to 2013….

Posted via web from Jen’s Posterous

Personal Responsibility for Health = Patriotism 2.0? Take Some Responsibility for Your Own Health. If Not For Yourself, Do it For Your Country.

December 31st, 2009 by admin

“If you eat too much, exercise too little, drink too much, smoke, take drugs, fail to wear a seat belt, or ignore gun safety, there’s only so much a doctor or hospital can do for you. And Americans do all those things, more than other people. And many are uncomfortably aware that self-destructive behavior is most often found among the poor and among minorities. Public policy can achieve only a limited impact against these problems. We’ll have to rethink the deeper structure of American food policy: subsidies to corn and soybean growers, the paving over of exurban land that might provide nearby cities with less expensive fruits and vegetables. Ultimately, though, these are decisions that individuals must make for themselves. The present concept of medicalized health care sends some unwelcome messages. By outsourcing the concept of health as something that doctors, hospitals, and now government do for you — rather than something that depends considerably on your own choices and efforts — we ask the medical system to do more than any medical system can do. As you consider your new year’s resolutions, remember: better habits will benefit not only your family and yourself — but all your neighbors and countrymen as well.”  

From: “Wednesday, December 30, 2009 | DCPCA Health News Alert.”

Posted via web from Jen’s Posterous

Kidney International – Table of Contents alert Volume 77 Issue 2

December 30th, 2009 by admin

KIDNEY INTERNATIONAL

January 2010 Volume 77 Number 2, pp 79 – 168

International Society of Nephrology
===================================
Your contribution is essential:
Renew your ISN membership now!
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IN THIS ISSUE
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In this Issue
http://links.ealert.nature.com/ctt?kn=45&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

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JOURNAL CLUB
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Journal Club
http://links.ealert.nature.com/ctt?kn=51&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

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COMMENTARIES
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Comorbidity and confounding in end-stage renal disease
Stephen L Seliger
Abstract: http://links.ealert.nature.com/ctt?kn=53&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=54&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

Hydrogen: another gas with therapeutic potential
James F George and Anupam Agarwal
Abstract: http://links.ealert.nature.com/ctt?kn=56&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=47&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

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MINI REVIEWS
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Linking an insect enzyme to hypertension: angiotensin II-epoxide hydrolase interactions
Ding Ai, John Y-J Shyy and Yi Zhu
Abstract: http://links.ealert.nature.com/ctt?kn=48&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=49&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

Parathyroid hormone measurement in CKD
Jean-Claude P Souberbielle, Hubert Roth and Denis P Fouque
Abstract: http://links.ealert.nature.com/ctt?kn=15&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=14&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

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ORIGINAL ARTICLES
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Oral hydrogen water prevents chronic allograft nephropathy in rats
Jon S Cardinal, Jianghua Zhan, Yinna Wang, Ryujiro Sugimoto, Allan Tsung, Kenneth R McCurry, Timothy R Billiar and Atsunori Nakao
Abstract: http://links.ealert.nature.com/ctt?kn=13&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=12&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

Inhibition of calcineurin phosphatase promotes exocytosis of renin from juxtaglomerular cells
Kirsten Madsen, Ulla G Friis, Jennifer L Gooch, Pernille B Hansen, Lisbeth Holmgaard, Ole Skott and Boye L Jensen
Abstract: http://links.ealert.nature.com/ctt?kn=11&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=9&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

Phosphoinositol 3-kinase-[gamma] mediates antineutrophil cytoplasmic autoantibody-induced glomerulonephritis
Adrian Schreiber, Susanne Rolle, Ludmilla Peripelittchenko, Joerg Rademann, Wolfgang Schneider, Friedrich C Luft and Ralph Kettritz
Abstract: http://links.ealert.nature.com/ctt?kn=74&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=73&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

Cyclic nucleotide signaling in polycystic kidney disease
Xiaofang Wang, Christopher J Ward, Peter C Harris and Vicente E Torres
Abstract: http://links.ealert.nature.com/ctt?kn=77&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=76&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

An improved comorbidity index for outcome analyses among dialysis patients
Jiannong Liu, Zhi Huang, David T Gilbertson, Robert N Foley and Allan J Collins
Abstract: http://links.ealert.nature.com/ctt?kn=64&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=65&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

Mycophenolate mofetil and intravenous cyclophosphamide are similar as induction therapy for class V lupus nephritis
Jai Radhakrishnan, Dimitrios-Anestis Moutzouris, Ellen M Ginzler, Neil Solomons, Ilias I Siempos and Gerald B Appel
Abstract: http://links.ealert.nature.com/ctt?kn=66&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=67&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

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THE RENAL CONSULT
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Non-hepatitis virus associated mixed essential cryoglobulinemia
Nicholas M P Annear, H Terence Cook, Mark Atkins, Charles D Pusey and Alan D Salama
Abstract: http://links.ealert.nature.com/ctt?kn=68&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=69&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

———————-
LETTERS TO THE EDITOR
———————-
Regarding `Calcium channel blocker-induced chylous ascites in peritoneal dialysis'
Renee Graice and Joanne M Bargman
http://links.ealert.nature.com/ctt?kn=99&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

Response to: "Regarding `Calcium channel blocker-induced chylous ascites in peritoneal dialysis'"
Wei-Liang Chen and Yu-Tzu Tsao
http://links.ealert.nature.com/ctt?kn=96&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

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NEPHROLOGY IMAGE
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Intrathoracic kidney with right Bochdalek's hernia
Shiu-Dong Chung, Kuo-Hsin Chen, Ming-Kuen Lai, Hsiao-Chun Chang, Chao-Yuan Huang and Hong-Jeng Yu
http://links.ealert.nature.com/ctt?kn=92&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

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MAKE YOUR DIAGNOSIS
———————-
The Case | Best not shaken or stirred! Chronic lymphocytic leukemia and hyperkalemia
Rebecca M Smalley, Shelly Cook and Micah R Chan
http://links.ealert.nature.com/ctt?kn=90&m=34520843&r=MTc2MzAwNDQzNwS2&b=2&j=NjMzMTE2NTgS1&mt=1&rt=0

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to make sure that you are not left behind.

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A Cellular Response to Broken Chromosomes

December 29th, 2009 by admin

Science Daily features an article titled How Cells Handle Broken Chromosomes. Quoting from the article:

Scientists have discovered a novel cellular response towards persistent DNA damage: After being recognized and initially processed by the cellular machinery, the broken chromosome is extensively scanned for homology and the break itself is later tethered to the nuclear envelope. The researchers have uncovered a surprising feature of how DNA strand breaks can be handled.

The writer notes elaborate DNA repair systems involved in the repair of DNA double strand breaks (DSB). Researchers observed a “tethering” of yeast DSB to the nuclear envelope. It was speculated that this might prevent errors engendered by recombination. Rad51, sometimes dubbed recombinase, spreads over the chromosome containing the break during the repair process. Another protein, H2A.Z, is also essential for the activation of Rad51 and relocating DSBs to the nuclear envelope. When H2A.Z is not present in cells such cells are very sensitive to DSBs.

Going on the Calendar – Body Computing

December 29th, 2009 by admin

At the Body Computing conference we explore ways to expand the ability of technology to deliver information to consumers and get better outcomes. Can you put entertainment in? With the Internet, the draw is entertainment, and that can be very motivating. So the Beating Heart is totally for your kid to play with. You stick a patch that looks like this cool glowing heart on your chest, and it communicates the heart rate to the phone. It gives kids insight into what the heart rate is. They can send a photo of the latest teen idol and see what effect it has on their friend’s heart rate. We will have increasing capability of monitoring ourselves this way.

From: “Implant Wizard – Health Data Management.”

Fall 2010.

Posted via web from Jen’s Posterous

Jam Wif What U Got – What’s Next for Getupandmove.me?

December 29th, 2009 by admin

This guy is workin’ it riffing on Smule’s Leaf Trombone app – I want to be in THAT number.

Speaking of numbers, Getupandmove.me is now just about a month old.

A peek at our stats and what we’ve learned so far:

* 254 users
NOTE: This number = sad face + a big challenge for your creator team – How do we get new friends to move with us? More on how we plan to gain user traction anon, but if we were speaking in Pig Latin our answer might look something like “Acebookfay.”

* 463 challenges
NOTE: Confirming, again, initial ’superusers’ or hyperactive ‘parent’ nodes in our social graph really like to tell other users what to do, cough cough, motivate each other.

We figured out there are 2 basic species of “guammies” – those who like to be told what to do, and those who like to boss others around. Ahem. Guess which I am :) . Even more interesting, however, as we begin to track the spread of contagious challenges from an initial user ‘node’ is that sometimes Guammies who like to ‘be told’ LEVEL UP and become Guammies who motivate. Fascinating future implications for UX design.

* 208 completed challenges
NOTE: Another sad face. We want to stay above 50% completed challenges (where BOTH challenger and responder complete challenge by clicking on “Done my part”). I want us to hit 75%.

In looking at our insanely detailed weekly internal report card, we figured out our open challenge format (challenge anyone on Twitter without making sure they’re ‘logged in’ or signed up for GUAM) means we have a lot of ‘ghosts,’ or Twitter users who have been invited but haven’t logged in to GUAM. We’re working on an enrollment loop to reduce this.

In keeping with our Open Sesame commitment to conversing with you, our users, about where we’re all headed together, Andrey and I figured we’d offer a more basic, FAQ type update on what we think this strange trip is all about.

Please to excuse the ‘yelling’ in subheads…

Q: WHY WE STARTED WITH MICROFITNESS:

A: We specifically targeted the preventive/wellness end of the PHI spectrum that we feel is not currently addressed by CRM systems in place nor in direct-to-consumer personal logging or biometric (”me-tric”) tracking applications like DailyMile.com or LiveStrong.com.

There are a ton of apps out there that help you track things – but NOTHING out there (that we’ve seen) that successfully harnesses existing energy in your social networks.

In 140 characters: There are a ton of apps and services that track activities but none that really motivate you to do the things you want to track.

Some try to mask this by giving you graphs and stats. Graphs and numbers are not motivation. Your friends and family are motivation.

We want to connect you to them so you can make ‘healthy’ contagious, one microfitness challenge at a time.

WHAT’S THE GOAL?

The goal of Getupandmove.me (speaking like a true amateur scientist) is to motivate users to move by harnessing the cascade effect of ‘hyperactive’ or frenetic super user “node” individuals in existing social networks. I just learned this is called ’sociometrics.’ Woo hoo for continuing ed…

WHY DO YOU, THE FOUNDERS, THINK GUAM HAS THE POTENTIAL TO RENOVATE HOW WE MAKE DAILY HEALTH DECISIONS?

One of the most fascinating things about guammies is that you’re not only taking responsibility for your OWN health by committing to a small act of fitness, but you are, in essence, *also* taking responsibility to ‘coach’ or ‘mentor’ another user to get up and move it by ‘bartering’ your fitness for theirs.

I’ve heard a lot of chatter from the bigwigs in healthcare this year about “getting consumers to take responsibility for their own health and wellbeing” blah blah blah.

You, my dear guammies, are not just talking or tweeting about this – you are DOING it. One challenge at a time.

It’s important here to look at how current health and wellness apps fail to use the most motivating things in your life – friends and family you ALREADY connect with in your social graph – to help you make better choices.

Motivation isn’t a set of instructions about how to do a pushup in perfect form.

Motivation is someone getting you interested in doing a friggin’ pushup in the first place, and we think meaningful, personally relevant motivation structures are the most critical element missing from the current wellness spectrum of applications.

WHY WE LOVE OUR LEAD USERS OR “RECRUITERS:”

We rely on our lead users to incentivize responders, at least for now. If you are a lead user – wow. Our hats are tipped to you.

Send the funniest or weirdest #getupandmove challenge (keep it clean people!) and we may get you a nifty tee shirt :) .

Our early lead users (”challengers”) provide social motivation not only to move themselves, but also in effect take responsibility for getting other users (”responders”) motivated and stay on track in that ’space between’ home and work where you may choose to do things OTHER than go for a run or go to the gym.

WHAT KIND OF NIFTY RELATIONSHIP GRAPHING WILL YOU DO TO STUDY SOCIAL CONTAGION?

Frankly, we don’t have a GD clue.

Well, I’m being modest, we have a few ideas, but nothing concrete at this early stage.

We’re examining all kinds of analytics and graphing APIs.

At minimum, we’re trying to see if it’s possible for your social graph to help inject small bits and bytes of microfitness into your daily life by tracking the ‘path’ of a challenge from user to user to new user etc, but even we aren’t sure yet how to track the contagion of movement motivation from ‘parent’ node users to ‘child’ responders.

WHY WE PUFFY HEART OUR RESPONDERS TOO:

Although we’re kind of relying on the strong backs of frenetically active challengers to motivate the guammie community, we are also very deliberately targeting responders usually are not initial or early adopters with our easy to use challenge formats.

If a challenger’s challenge fell in the forest, and nobody heard it, would it still make a sound? Nope.

Responders – you are the heartbeat of #getupandmove. You’re going out on a limb. You’re doing a favor for a friend that asks you to move. And you’re motivating others in return.

We salute you!

WHY DO YOU THINK THIS MICROFITNESS JAZZ MAY WORK?

We’re keeping challenges small and simple – users don’t have to be a pro athlete or marathoner to use Get Up and Move.

We’re not competing with sites like DailyMile.com and Skimple or RunThere to log what you’ve done…

We’re big fans. In fact, eventually we want our users to move from ‘microfitness’ at the daily level using Get Up and Move to larger macro challenges like training for a marathon using DailyMile.com.

We aren’t enabling logging functions – instead the most primary function of GUAM is that “guammies” (getupandmove.me users) are motivating each other to move…if there are psychological or behavioral stages to working out, we’re in the “pre move” category.

And in the “pre move” category (as opposed to the “actively moving” category and the “I want to move MORE” category addressed by other apps and communities), the most important thing is someone providing that motivation for you to simply get up and do SOMETHING.

That’s where Getupandmove.me comes in.

IF I HAD TO TELL MY COUSIN/SPOUSE/EMPLOYER/3 YO/IMAGINARY FRIEND WHAT GUAM IS ABOUT IN 1 SENTENCE, WHAT WOULD I SAY?

At the most basic level, Getupandmove.me enables users to challenge friends and family to ‘microfitness’ challenges, in real time using simple preset options like “dance to 2 songs” or create your own using our free web app.

DO YOU SEE GUAM EXPANDING INTO THE MORE ‘MAINSTREAM’ HOSPITAL/HEALTHCARE MARKETS?

Sure.

For PHI (personal health information) tracking, the sky’s the limit.

Deploying something like Getupandmove.me for medication adherence, physical therapy exercise tracking, telehealth/home monitoring and self reporting of critical biometrics for, say, diabetes treatments would be awesome.

OK, BUT HOW ARE YOU GOING TO TRACK THAT STUFF? ANY NIFTY DEVICES LIKE FITBIT, NIKE+, ETC PLANNED?

We haven’t yet integrated with personal biometric trackers like FitBit or WakeMate (for sleep), but are considering it in the future – as well as telemedicine integration for challenges and self-reporting on metrics important for preventive health in conditions like diabetes, etc.

SO WHAT SNAZZY NEW FEATURES CAN WE LOOK FOR NEXT?

Planned future features include Facebook Connect integration (version 3.0, mid-January), group challenges, export activity to calorie counters, etc.

HOW THE HECK IS THIS GOING TO MAKE MOOLAH?

Our ambitious B2B enterprise market plan includes:

* gyms (reminders for new clients)
* events/conferences (onsite fitness challenges for attendees and exhibitors)
* colleges/high schools (for sports teams training in the pre or off seasons)
* hospitals/clinics (community wellness programs)
* medication/pharma reminders
* and employer wellness programs

There are a few other sectors we’re in the early stages of exploring viability.

Plus at some point soon we’ll be doing mobile schtuff.

That’s about it for now guammies.

Til next time, may the force be with you.

@jensmccabe
@shazow

PS – Be on the lookout for a new “Stealth Mode” feature release on Monday that protects your privacy to an uber-degree.

Posted via web from Get Up and Move!

Newly Discovered Gene Mutation Linked to Nerve Diseases

December 29th, 2009 by admin

Newly Discovered Gene Mutation Linked to Nerve Diseases

December 28, 2009 By Marla Paul <!–

–>

(PhysOrg.com) — Researchers from the Northwestern University Feinberg School of Medicine have identified mutations in the gene for TRPV4 that cause two related degenerative motor nerve disorders, scapuloperoneal spinal muscular atrophy (SPSMA) and hereditary motor and sensory neuropathy type IIC (CMT2C).

These disorders cause progressive weakness of the limbs, breathing muscles and vocal cords. The Northwestern University researchers also established that TRPV4 is involved in homeostasis of intracellular calcium during axonal activity. As a result, this discovery has wider implications for understanding non-genetic neuropathies and motor axon degeneration.

Han-Xiang Deng, MD, Ph.D., associate professor of neurology, and Teepu Siddique, MD, the Les Turner ALS Foundation/Herbert C. Wenske Professor, both of the Davee Department of Neurology and Clinical Neurosciences and their colleagues published these findings in the online edition of Dec. 27. Dr. Siddique is also a neurologist at Northwestern Memorial Hospital.

“Abnormally functioning TRPV4 receptors may drive excess calcium into neuronal extensions called axons,” said Siddique, who is also director of the Division of Neuromuscular Medicine at Northwestern’s Feinberg School of Medicine. “We think this may be a mechanism by which axons of motor or may be damaged in a variety of neurological conditions.”

The discovery adds a new dimension to scientists’ understanding of the way innervate muscle function. “We know an electrical impulse starts from the spinal cord and goes out to the muscle,” Deng said. “That is how things have been understood. Now we have this receptor that may modulate axonal activity, so, if it is not functioning properly, there may be nerve damage.”

Sididque noted that because TRPV4 receptors are activated by pressure, amongst other modalities, their malfunctioning could also be relevant to compression . He further said the role of TPRV4 in the pathophysiology of other spinal muscular atrophies and amyotrophic lateral sclerosis (ALS, also called Lou Gehrig disease) should also be investigated. ALS is a progressive and usually fatal neurodegenerative disease that affects nerve cells in the brain and spinal cord.

Robert Delong, MD, now professor emeritus of pediatrics at Duke University School of Medicine, and Siddique, first identified scapuloperoneal in a large New England family of French-Canadian origin in 1992. In 1994, Peter Dyck, MD, professor of neurology at the Mayo Clinic, identified the related disorder hereditary motor and sensory neuropathy type IIC in an American family of English and Scottish descent. In this new study, gene mapping and sequencing of DNA from these two families revealed the key TRPV4 gene mutations.

Provided by Northwestern University (news : web)

Nature Methods Contents: January 2010 Volume 7 pp 1 – 85

December 29th, 2009 by admin

NATURE METHODS

January 2010 Volume 7 Number 1, pp 1 – 85

Visit Nature Methods online to browse the journal.

Now available at http://links.ealert.nature.com/ctt?kn=165&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

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=====================================================================

———————-
SPECIAL FEATURE
———————-
Method of the Year 2009
Nature Methods' Method of the Year 2009 goes to induced pluripotency
for its potential for biological discovery. This series of
articles-and the related video-showcase how induced pluripotency
is coming into its own in 2009 as a tool for discovery in both basic
and disease biology and explore the incredible impact this area
promises to have in biological research. The Methods to Watch
feature provides a glimpse of future Methods of the Year and the
Reader's Choice shows methods nominated by readers and editors,
and the votes that they received.
http://links.ealert.nature.com/ctt?kn=169&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

———————-
EDITORIAL
———————-
Special feature: Method of the Year
Method of the Year 2009 p1
doi:10.1038/nmeth.f.294
The ability to return mature body cells to a pluripotent state has
wide-ranging potential as a tool for discovery in both disease and
basic biology.
http://links.ealert.nature.com/ctt?kn=111&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

———————-
THIS MONTH
———————-
The author file p3
doi:10.1038/nmeth0110-03
Supercharged SRM: synthetic peptides bring high-throughput
assays to targeted proteomics.
http://links.ealert.nature.com/ctt?kn=113&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

———————-
CORRESPONDENCE
———————-
A red-shifted Renilla luciferase for transient reporter-gene
expression pp5 – 6
Andreas Markus Loening, Anca Dragulescu-Andrasi and Sanjiv
Sam Gambhir
doi:10.1038/nmeth0110-05
http://links.ealert.nature.com/ctt?kn=110&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Restricted ethnic diversity in human embryonic stem cell
lines pp6 – 7
Louise C Laurent et al.
doi:10.1038/nmeth0110-06
http://links.ealert.nature.com/ctt?kn=66&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

———————-
RESEARCH HIGHLIGHTS
———————-
The mobile microscope p9
Daniel Evanko
doi:10.1038/nmeth0110-09
A miniature head-mounted two-photon microscope small enough
for a rat to carry allows researchers to visualize neuronal signaling
while the animal freely interacts with its environment.
http://links.ealert.nature.com/ctt?kn=68&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Bring on the promoters pp10 – 11
Nicole Rusk
doi:10.1038/nmeth0110-10a
High-throughput saturation mutagenesis determines the contribution
of each base in a core promoter to overall promoter strength.
http://links.ealert.nature.com/ctt?kn=71&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

The importance of being negative pp10 – 11
Allison Doerr
doi:10.1038/nmeth0110-10b
The Negatome is a database of non-interacting protein pairs that can
be used for training protein-protein interaction prediction
algorithms.
http://links.ealert.nature.com/ctt?kn=73&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

News in brief p11
doi:10.1038/nmeth0110-11
http://links.ealert.nature.com/ctt?kn=75&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Caught in action p12
Michael Eisenstein
doi:10.1038/nmeth0110-12
Screening reveals a chemical activator that triggers apoptosis by
locking inactive but dynamic proenzymes into a more active state,
suggesting a promising strategy for targeting proteases.
http://links.ealert.nature.com/ctt?kn=77&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

A family tree in a tumor p14
Monya Baker
doi:10.1038/nmeth0110-14
A new technique finds genomic subpopulations to indicate cancer
progression.
http://links.ealert.nature.com/ctt?kn=58&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

———————-
NEWS FEATURE
———————-
Special feature: Method of the Year
iPS cells: potent stuff pp17 – 19
Monya Baker
doi:10.1038/nmeth.f.281
Now that the generation of induced pluripotent stem cells is becoming
routine, researchers can get on to the more exciting prospect of
using the cells to make discoveries in disease and basic biology.
Monya Baker reports.
http://links.ealert.nature.com/ctt?kn=61&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

———————-
PRIMER
———————-
Special feature: Method of the Year
Primer: induced pluripotency pp20 – 21
Natalie de Souza
doi:10.1038/nmeth.f.293
A brief overview of methods for reprogramming to induced pluripotency
and of the properties of induced pluripotent stem cells.
http://links.ealert.nature.com/ctt?kn=62&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

———————-
COMMENTARIES
———————-
Special feature: Method of the Year
The mysteries of induced pluripotency: where will they
lead? pp22 – 24
Andras Nagy and Kristina Nagy
doi:10.1038/nmeth.f.292
The discovery that it is possible to render somatic cells pluripotent
by the exogenous expression of a set of transcription factors
provides an experimental model for studying the molecular nature
of cellular identity.
http://links.ealert.nature.com/ctt?kn=63&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Special feature: Method of the Year
Induced pluripotent stem cell technology for the study of human
disease pp25 – 27
Gabsang Lee and Lorenz Studer
doi:10.1038/nmeth.f.283
iPS cell technology makes patient- and disease-specific human
cells widely available. While technical challenges still remain,
the use of these tools will greatly expand our understanding
of human disease.
http://links.ealert.nature.com/ctt?kn=83&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Special feature: Method of the Year
Stem cell research policy and iPS cells pp28 – 33
Timothy Caulfield et al.
doi:10.1038/nmeth.f.282
The field of induced pluripotent stem cells (iPSCs) will be subject
to a wide range of laws and research ethics policies, many of
which exist as a result of the controversies associated with research
on human embryonic stem cells. Understanding this potentially
complex regulatory environment will help iPSC research move
forward and will inform future policy.
http://links.ealert.nature.com/ctt?kn=81&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

———————-
METHODS TO WATCH
———————-
Special feature: Method of the Year
Targeted proteomics p34
Allison Doerr
doi:10.1038/nmeth.f.284
Technology for sensitively and reproducibly detecting targeted
proteins by mass spectrometry picks up speed.
http://links.ealert.nature.com/ctt?kn=82&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Special feature: Method of the Year
Optical imaging of the native brain p34
Daniel Evanko
doi:10.1038/nmeth.f.285
Methodological developments are opening the functioning brain to
cellular-level investigation using light.
http://links.ealert.nature.com/ctt?kn=87&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Special feature: Method of the Year
Mapping genomes in 3D p35
Nicole Rusk
doi:10.1038/nmeth.f.286
Refinements in methods to uncover the higher-order structure of
the genome will allow functional insight into genomic architecture
at high resolution.
http://links.ealert.nature.com/ctt?kn=88&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Special feature: Method of the Year
Single-cell methods p35
Natalie de Souza
doi:10.1038/nmeth.f.287
The ability to study single cells will permit a better understanding
of cellular heterogeneity.
http://links.ealert.nature.com/ctt?kn=84&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Special feature: Method of the Year
Label-free microscopy p36
Daniel Evanko
doi:10.1038/nmeth.f.288
New methods to coax signals from unlabeled biological molecules
may finally fulfill the promise of practical label-free microscopy
with molecular specificity.
http://links.ealert.nature.com/ctt?kn=85&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Special feature: Method of the Year
High-throughput phenotyping p36
Natalie de Souza
doi:10.1038/nmeth.f.289
Automated methods to score phenotypes in model organisms continue
to develop and will permit previously inaccessible areas of biology
to be probed.
http://links.ealert.nature.com/ctt?kn=79&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Special feature: Method of the Year
Synthetic life p37
Allison Doerr
doi:10.1038/nmeth.f.290
Will new methods and an emerging understanding of the minimal
requirements for cellular life be sufficient to construct a synthetic
organism?
http://links.ealert.nature.com/ctt?kn=80&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Special feature: Method of the Year
A direct view of the fifth base p37
Nicole Rusk
doi:10.1038/nmeth.f.291
Will some single molecule sequencing strategies be able to deliver
on the promise of direct methyl cytosine sequencing?
http://links.ealert.nature.com/ctt?kn=78&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

———————-
NEWS AND VIEWS
———————-
Reduce, reuse, reprogram pp39 – 40
Thomas P Zwaka
doi:10.1038/nmeth0110-39
Mouse lines with inducible reprogramming factors expressed from
a single genomic locus will allow reprogramming studies in multiple
cell types and defined genetic backgrounds.
http://links.ealert.nature.com/ctt?kn=205&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Sorting cardiomyocytes: a simple solution after all? pp40 – 42
Christine Mummery
doi:10.1038/nmeth0110-40
Cardiomyocytes can be sorted to high purity upon staining them with
a dye that labels mitochondria. This permits the preparation of pure
populations of cardiomyocytes differentiated from stem cells.
http://links.ealert.nature.com/ctt?kn=181&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

———————-
BRIEF COMMUNICATIONS
———————-
High-throughput generation of selected reaction-monitoring assays
for proteins and proteomes pp43 – 46
Paola Picotti et al.
doi:10.1038/nmeth.1408
Selected reaction monitoring (SRM) is a powerful mass spectrometry
technology to reliably detect selected protein targets, even those at
very low abundance, but requires tedious assay development for
each protein of interest. High-throughput SRM assay development
is now possible by using crude synthetic peptide libraries without
purification to represent each protein target.
Abstract: http://links.ealert.nature.com/ctt?kn=180&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=185&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Chromatin profiling by directly sequencing small quantities of
immunoprecipitated DNA pp47 – 49
Alon Goren et al.
doi:10.1038/nmeth.1404
Single-molecule sequencing of poly(A)-tailed chromatin
immunoprecipitated DNA proves equal in sensitivity and accuracy
to amplification-based sequencing technologies and allows analysis
of samples sizes as small as 50 pg.
Abstract: http://links.ealert.nature.com/ctt?kn=184&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=187&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Floxin, a resource for genetically engineering mouse
ESCs pp50 – 52
Veena Singla et al.
doi:10.1038/nmeth.1406
An efficient system for the reversion and modification of mouse
gene trap alleles is presented. It is applicable to available
collections of gene trap embryonic stem cell lines.
Abstract: http://links.ealert.nature.com/ctt?kn=186&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=189&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

A reprogrammable mouse strain from gene-targeted embryonic stem
cells pp53 – 55
Matthias Stadtfeld, Nimet Maherali, Marti Borkent and Konrad
Hochedlinger
doi:10.1038/nmeth.1409
A mouse strain in which cellular reprogramming factors are expressed
from a defined genomic locus is presented. It will enable studies of
reprogramming in multiple cell types as well as facilitate
comparisons between induced pluripotent stem cells and embryonic
stem cells. Also in this issue, a paper by Carey et al. presents
related tools.
Abstract: http://links.ealert.nature.com/ctt?kn=188&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=176&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Single-gene transgenic mouse strains for reprogramming adult somatic
cells pp56 – 59
Bryce W Carey et al.
doi:10.1038/nmeth.1410
Mouse strains in which three or four cellular reprogramming factors
are expressed from a defined genomic locus are presented. They will
enable studies of reprogramming in multiple cell types as well as
facilitate comparisons between induced pluripotent stem cells and
embryonic stem cells. Also in this issue, a paper from Stadtfeld
et al. presents related tools.
Abstract: http://links.ealert.nature.com/ctt?kn=57&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=59&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

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=====================================================================

———————-
ARTICLES
———————-
Nongenetic method for purifying stem cell-derived
cardiomyocytes pp61 – 66
Fumiyuki Hattori et al.
doi:10.1038/nmeth.1403
Staining with a mitochondrial dye permits high-purity isolation of
cardiomyocytes from embryonic and induced pluripotent stem cells
of several species, without genetic modification.
Abstract: http://links.ealert.nature.com/ctt?kn=74&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=76&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Engineering a polarity-sensitive biosensor for time-lapse imaging of
apoptotic processes and degeneration pp67 – 73
Yujin E Kim, Jeannie Chen, Jonah R Chan and Ralf Langen
doi:10.1038/nmeth.1405
A polarity-sensitive annexin-based biosensor called pSIVA becomes
strongly fluorescent only after reversibly binding to the plasma
membrane. pSIVA allows live-cell imaging of the apoptotic process
in degenerating neurons in vitro and in vivo.
Abstract: http://links.ealert.nature.com/ctt?kn=69&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=72&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

Integrated analysis of receptor activation and downstream signaling
with EXTassays pp74 – 80
Anna Botvinnik, Sven P Wichert, Tobias M Fischer and Moritz J Rossner
doi:10.1038/nmeth.1407
By combining a protein complementation assay with a transcriptional
reporter assay based on short expressed oligonucleotide tags (EXTs),
the authors monitor tyrosine kinase receptor dimerization in
conjunction with effector recruitment and downstream signaling.
Abstract: http://links.ealert.nature.com/ctt?kn=65&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=67&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

———————-
TECHNOLOGY FEATURE
———————-
IPSCs: One cell to rule them all? pp81 – 85
Michael Eisenstein
doi:10.1038/nmeth0110-81
Rapid progress with induced pluripotent stem cells is bringing
scientists closer to understanding their strengths and weaknesses
as embryonic stem cell stand-ins.
http://links.ealert.nature.com/ctt?kn=64&m=34519504&r=MTc2NjExMzUwMAS2&b=2&j=NjMyNzg0OTMS1&mt=1&rt=0

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Immuno-epigenetics

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This special collection brings together articles from Nature, Nature Immunology, Nature Reviews Immunology and Nature Reviews Drug Discovery that have contributed to advances and discussions in the field of immune cell epigenetics.

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Laboratory Investigation – Table of Contents alert Volume 90 Issue 1

December 29th, 2009 by admin

LABORATORY INVESTIGATION

January 2010 Volume 90 Number 1, pp 2 – 139

———————————————————————

Laboratory Investigation – dedication to the publication of current
research that significantly advances the understanding of human
and experimental disease. Particular emphasis is given to
original research exploring the pathobiology of disease, including
insights gained into the structural and molecular pathogenesis of
disease, and the biological basis for morphologic manifestations
of disease. Visit the journal online to learn more
http://links.ealert.nature.com/ctt?kn=47&m=34519494&r=MTc2Mjk0MzQ4MgS2&b=2&j=NjMyNzgzMDgS1&mt=1&rt=0

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*2008 Journal Citation Report (Thomson Reuters 2009)

———————-
INSIDE LI
———————-
Inside Lab Invest
http://links.ealert.nature.com/ctt?kn=31&m=34519494&r=MTc2Mjk0MzQ4MgS2&b=2&j=NjMyNzgzMDgS1&mt=1&rt=0

———————-
EDITORIALS
———————-
What editors want in an abstract
Catherine M Ketcham, Robert W Hardy, Brian Rubin and Gene P Siegal
http://links.ealert.nature.com/ctt?kn=55&m=34519494&r=MTc2Mjk0MzQ4MgS2&b=2&j=NjMyNzgzMDgS1&mt=1&rt=0

Attacking the source: anti-PDX-1 responses in type 1 diabetes
Yaima Luzardo and Clayton Elwood Mathews
Abstract: http://links.ealert.nature.com/ctt?kn=56&m=34519494&r=MTc2Mjk0MzQ4MgS2&b=2&j=NjMyNzgzMDgS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=57&m=34519494&r=MTc2Mjk0MzQ4MgS2&b=2&j=NjMyNzgzMDgS1&mt=1&rt=0

———————-
RESEARCH ARTICLES
———————-
FTY720, a sphingosine 1-phosphate receptor modulator, inhibits
CD1d-restricted NKT cells by suppressing cytokine production but not migration
Su Jin Hwang, Ji Hyung Kim, Hye Young Kim, Sanghee Kim and Doo Hyun Chung
Abstract: http://links.ealert.nature.com/ctt?kn=58&m=34519494&r=MTc2Mjk0MzQ4MgS2&b=2&j=NjMyNzgzMDgS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=50&m=34519494&r=MTc2Mjk0MzQ4MgS2&b=2&j=NjMyNzgzMDgS1&mt=1&rt=0

Rho/ROCK and MEK/ERK activation by transforming growth factor-[alpha]
induces articular cartilage degradation
C Thomas G Appleton, Shirine E Usmani, John S Mort and Frank Beier
Abstract: http://links.ealert.nature.com/ctt?kn=53&m=34519494&r=MTc2Mjk0MzQ4MgS2&b=2&j=NjMyNzgzMDgS1&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=35&m=34519494&r=MTc2Mjk0MzQ4MgS2&b=2&j=NjMyNzgzMDgS1&mt=1&rt=0

Pancreatic duodenal homeobox 1 protein is a novel [beta]-cell-specific
autoantigen for type I diabetes
Shi-Wu Li, Vijay Koya, Yi Li, William Donelan, Peng Lin, Westley H Reeves
and Li-Jun Yang
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Erythropoietin-induced upregulation of endothelial nitric oxide synthase
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Martin Rucker, Mickael Tobalem, Brigitte Vollmar, Michael D Menger
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Acyclic retinoid inhibits angiogenesis by suppressing the MAPK pathway
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Isolation of targeting nanobodies against co-opted tumor vasculature
Ilse Roodink, Maarten Franssen, Malou Zuidscherwoude, Kiek Verrijp,
Tom van der Donk, Jos Raats and William PJ Leenders
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Effects of sulfur dioxide on hypoxic pulmonary vascular structural remodeling
Yan Sun, Yue Tian, Mainali Prabha, Die Liu, Stella Chen, Rongyuan Zhang,
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Nephron-deficient Fvb mice develop rapidly progressive renal failure
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Youli Wang, Kathleen O Heilig, Andrew W Minto, Shenglin Chen,
Minghui Xiang, David A Dean, Richard C Geiger, Anthony Chang,
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Intrarenal expression of microRNAs in patients with IgA nephropathy
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Curcumin limits the fibrogenic evolution of experimental steatohepatitis
Francesco Vizzutti, Angela Provenzano, Sara Galastri, Stefano Milani,
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Umberto Arena, Giacomo Laffi, Maurizio Parola, Massimo Pinzani and Fabio Marra
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Inhibition of nitric oxide synthesis during induced cholestasis ameliorates
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Laura M Lopez-Sanchez, Fernando J Corrales, Montserrat Barcos, Isabel Espejo,
Juan R Munoz-Castaneda and Antonio Rodriguez-Ariza
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Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related
SIRS due to the release of preformed stores in the intestine
R Britt McILwain, Joseph G Timpa, Ashish R Kurundkar, David W Holt, David R Kelly,
Yolanda E Hartman, Mary Lauren Neel, Rajendra K Karnatak, Robert L Schelonka,
G M Anantharamaiah, Cheryl R Killingsworth and Akhil Maheshwari
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Interested in e-Prescribing? Keep an Eye on Scotland’s CMS Program this Spring…

December 28th, 2009 by admin

The CMS offers patients with LTCs the chance to benefit from ‘pharmaceutical care planning’ with their community pharmacist as well as shared care and repeat dispensing.

When a patient signs up for the CMS the pharmacy’s patient medication record will send an electronic notification to the GP’s IT system which then allows the GP to choose whether to enter into a shared care agreement with the option to generate serial prescriptions for up to 48 weeks.

The pharmacist draws up a pharmaceutical care plan with the patient and if a shared care agreement is in place relevant information shared between the pharmacy and the GP with informed patient consent. At the end of the serial prescription time period the pharmacist sends an electronic end of care treatment summary and a request for a new serial prescription.

From: “E-Health Insider Primary Care :: Scotland’s CMS to go national in April.”

Posted via web from Jen’s Posterous

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